The Pfu DNA Polymerase is a high-fidelity DNA polymerase that is widely used in various molecular biology applications. It is derived from the thermophilic bacterium Pyrococcus furiosus and exhibits proofreading activity, resulting in a low error rate during DNA synthesis. The Pfu DNA Polymerase is known for its ability to amplify long DNA fragments with high accuracy and efficiency.
Advantages:
High fidelity: Pfu DNA Polymerase has superior proofreading properties compared to other thermostable polymerases. It possesses 3’ to 5’ exonuclease proofreading activity, which removes nucleotides misincorporated at the 3’ end of the growing DNA strand. This results in a lower error rate during DNA synthesis.
Amplification of long DNA fragments: Pfu DNA Polymerase is known for its ability to amplify long DNA fragments with high accuracy and efficiency. It is commonly used for applications that require high-fidelity DNA synthesis, such as cloning, site-directed mutagenesis, and sequencing.
Blunt-ended PCR products: Pfu DNA Polymerase generates blunt-ended PCR products. This makes it ideal for certain applications, such as TA cloning.
Compatibility with other polymerases: Pfu DNA Polymerase can be used in conjunction with other polymerases, such as Taq polymerase, to obtain the fidelity of Pfu with the speed of Taq polymerase activity.
Result
Fig: Gene was amplified and loaded on 2% agarose gel. Lane 3 represent the amplification using Pfu DNA Polymerase from Gene to protein Pvt Ltd.
