Pet 21b Plasmid DNA

Description

pET-21b (+) is a bacterial vector primarily used for cloning and expression of recombinant proteins in E. coli. It contains the necessary elements for efficient expression of the target protein, including a strong T7 promoter, a ribosome binding site, and a 6xHis-tag for purification.

Applications:

• Expression and purification of recombinant proteins in E. coli: pET-21b (+) is widely used for the expression and purification of recombinant proteins in E. coli. The T7 promoter allows for efficient transcription of the target gene, while the 6xHis-tag facilitates purification of the expressed protein.
• Cloning and subcloning of DNA fragments: pET-21b (+) can be used for cloning and subcloning of DNA fragments. The vector’s multiple cloning sites enable the insertion of DNA fragments into the plasmid for further analysis or manipulation.
• Mutagenesis and site-directed mutagenesis: pET-21b (+) can also be employed for mutagenesis and site-directed mutagenesis experiments. Researchers can introduce specific mutations or modifications into the target gene using techniques such as PCR-based mutagenesis or site-directed mutagenesis.