Description:
Nhe I is a type II restriction enzyme that recognizes the DNA sequence 5′-G/CTAGC-3′ and cuts between the two C residues, generating DNA fragments with 5′-cohesive termini. It is commonly used in molecular biology research to manipulate DNA fragments. Nhe I is supplied with 10 U/µL concentration and is available commercially.
Recognition: G↑CTAGC
Sequence: CGATC↓G
Applications:
- Molecular cloning: Nhe I is commonly used in molecular cloning techniques to insert genes into plasmids.
- Restriction site mapping: Nhe I can be used to map restriction sites on DNA molecules.
- Genotyping: Nhe I can be used for genotyping studies to identify genetic variations.
- Southern blotting: Nhe I can be used in Southern blotting techniques to detect specific DNA sequences.
- Restriction fragment length polymorphism (RFLP): Nhe I can be used in RFLP analysis to study genetic variations.
- SNP analysis: Nhe I can be used to analyze single nucleotide polymorphisms (SNPs).
