G2P T7 RNA Polymerase
Highlights
- High-efficiency RNA synthesis
- Suitable for in vitro transcription and probe generation
- Supplied in a stable, ready-to-use format
Product Details
G2P T7 RNA Polymerase is a recombinant DNA-dependent RNA polymerase derived from the T7 bacteriophage. It catalyzes the synthesis of RNA in the 5′→3′ direction using linear or plasmid DNA templates containing a T7 promoter. This enzyme is widely used for in vitro mRNA synthesis, radiolabeled RNA probe generation, antisense RNA production, and sgRNA synthesis for CRISPR applications. It is supplied in a glycerol-containing buffer for enhanced stability and is ready for use in standard transcription reactions.
Kit Contents
| Component | Volume |
|---|---|
| T7 RNA Polymerase (100 U/μL) | 100 μL (10,000U) |
| 10X Transcription Buffer | 100 μL |
Additional Information
- Store at −20°C
- Make working aliquots to reduce freeze–thaw cycles
- Mix gently before use; do not vortex
