Compli-AmpTM High fidelity Two-step RT-PCR Kit
Highlights
- Two-step protocol with separate RT and PCR phases
- High sensitivity and full-length cDNA synthesis
- Suitable for probe- or dye-based qPCR
Product Details
Compli-AmpTM combines MMLV RT and Pfu polymerase for high-fidelity RT-PCR. Ideal for gene expression studies where control over RT and PCR steps is preferred. Works efficiently on low-copy targets and long transcripts.
Kit Contents
| Component | RNA007-50 | RNA007-100 |
|---|---|---|
| RNase Inhibitor (40 U/μL) | 12.5 μL | 25 μL |
| Oligo dT20 (50 μM) | 25 μL | 50 μL |
| Random Nonamer (50μM) | 25 μL | 50 μL |
| dNTP (10 mM) | 50 μL | 100 μL |
| RT Buffer (10X) | 100 μL | 200 μL |
| MMLV-RT Enzyme (80U/μL) | 12.5 μL | 25 μL |
| Pfu Polymerase (5 U/μL) | 12.5 μL | 25 μL |
| Pfu Polymerase (5 U/μL) | 150 μL | 300 μL |
| Nuclease-Free Water | 1 mL | 2 mL |
Additional Information
- Storage: –20°C
