Description:
Tris-HCl buffer is a commonly used buffering solution in molecular biology labs. It is used to control the acidity and osmolarity of a solution. Tris-HCl is a buffering system that has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics, and molecular biology.
Applications:
- Biochemical research: Tris-HCl buffer is used in various biochemical research experiments such as western blotting, biochips, biological hybridization, and in vitro diagnostics.
- Protein purification: Tris-HCl buffer is used as a component of lysis buffers and wash buffers during protein purification processes.
- Cell culture: Tris-HCl buffer is used as a component of cell culture media to maintain the pH and osmolarity of the solution.
- Sample storage: Tris-HCl buffer is used for the storage of biological samples to maintain their stability and prevent degradation.